Rapid Identification of the α-Glucosidase Inhibitory Compounds from Thunberg’s Geranium (Geranium thunbergii Sieb. et Zucc.)

저자

신*현

게재저널

키워드

diabetes, Geranium thunbergii, geraniin, HPLC

개요

In the current study, our investigation of inhibitory compounds from the ethyl acetate-soluble fraction of 95% ethanol extract of Thunberg’s Geranium (Geranium thunbergii Sieb. et Zucc.; TG) was followed by identification of the
inhibitory compounds by a combination of HPLC microfractionation and an enzyme assay in a 96-well plate.

Structural analyses of the active compounds were carried out using LC-MSn. The main compound inhibiting yeast α-glucosidase in this plant was tentatively identified as geraniin by LC-MSn. Six compounds, p-hydroxybenzoic acid (1)
(IC50 >362.32 μM), brevifolin carboxylic acid (2) (IC50> 171.23 μM), geraniin (3) (IC50 =4.09 μM), ellagic acid (4) (IC50 =63.91 μM), kaempferol-3-O-arabinofuranosyl-7-Orhamnopyranoside (5) (IC50 =27.70 μM), and kaempferitrin (6) (IC50 >86.51 μM) were isolated from the active EtOAcsoluble
fraction of TG on yeast α-glucosidase. However, the 6 isolated compounds were very weak inhibitory activity against mammalian α-glucosidase and α-amylase.

This study suggests that the developed HPLC microfractionation with an enzyme assay system is one of the powerful tools for rapid screening and identification of enzyme inhibitors in complex extracts.

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• 42. 신국현_현초(alpha-glucosidase논문)-FBS2.pdf [881,137 byte]

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